Journal: AMB Express

Article Title: Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri

doi: 10.1186/2191-0855-1-37

Figure Lengend Snippet: Primers and peptide sequences used in this work

Article Snippet: Primers were procured from VBC-Biotech (Austria) and the inducer sakacin P induction peptide (SppIP) was synthesized from GenScript (USA).

Techniques: Sequencing

Journal: AMB Express

Article Title: Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri

doi: 10.1186/2191-0855-1-37

Figure Lengend Snippet: SDS-PAGE analysis of L. reuteri whole cell lysates for yqhD expression . Lane 2 untransformed L. reuteri , lane 3 uninduced recombinant L. reuteri HR2, lane 4 recombinant 5 h after induction with SppIP, lanes 1 & 5, protein molecular weight marker.

Article Snippet: Primers were procured from VBC-Biotech (Austria) and the inducer sakacin P induction peptide (SppIP) was synthesized from GenScript (USA).

Techniques: SDS Page, Expressing, Recombinant, Molecular Weight, Marker

Journal: Bioactive Materials

Article Title: A ROS-responsive hydrogel incorporated with dental follicle stem cell-derived small extracellular vesicles promotes dental pulp repair by ameliorating oxidative stress

doi: 10.1016/j.bioactmat.2024.06.036

Figure Lengend Snippet: DFSC-sEVs enhanced the survival, proliferation and osteogenesis/odontogenesis of H 2 O 2 -damaged DPSCs. (A) DPSCs apoptosis was examined by an Annexin V-FITC/7AAD assay. (B) Apoptosis rates of DPSCs, including early apoptosis (Annexin-V + 7AAD − ) and late apoptosis (Annexin-V + 7AAD + ). (C, D) Representative images of BrdU staining and quantification of DPSCs. (E) Representative images of alizarin red staining after osteogenic induction for 21 d. (F) qRT‒PCR analysis of the mRNA expression of the odontogenesis-related factors Alpl , Ibsp and Dcn . (G) Representative images of Oil Red O staining after adipogenic induction for 21 d. (H) qRT‒PCR analysis of the mRNA expression of the adipogenic-related factors Cfd , Cebpa and Cebpb . *** P < 0.001, ** P < 0.01, * P < 0.05.

Article Snippet: The osteogenic differentiation of DFSCs and DPSCs was induced with osteogenic induction media for 21 days according to the manufacturer's instructions (Cyagen Biosciences, China), after which alizarin red staining was performed to visualize calcium deposits.

Techniques: BrdU Staining, Staining, Expressing

Journal: Bioactive Materials

Article Title: A ROS-responsive hydrogel incorporated with dental follicle stem cell-derived small extracellular vesicles promotes dental pulp repair by ameliorating oxidative stress

doi: 10.1016/j.bioactmat.2024.06.036

Figure Lengend Snippet: DFSC-sEVs exerted cytoprotective effects on H 2 O 2- induced damage by maintaining mitochondrial homeostasis. (A) DPSCs apoptosis was examined using an Annexin V-FITC/7AAD assay. (B) Apoptosis rates of DPSCs, including early apoptosis (Annexin-V + 7AAD − ) and late apoptosis (Annexin-V + 7AAD + ). (C, D) Representative images of BrdU staining and quantification of DPSCs. (E) Representative images of alizarin red staining after osteogenic induction for 21 d. (F) qRT‒PCR analysis of the mRNA expression of the odontogenesis-related factors Alpl , Ibsp and Dcn . (G) Representative images of Oil Red O staining after adipogenic induction for 21 d. (H) qRT‒PCR analysis of the mRNA expression of the adipogenic-related factors Cfd , Cebpa and Cebpb . *** P < 0.001, ** P < 0.01, * P < 0.05.

Article Snippet: The osteogenic differentiation of DFSCs and DPSCs was induced with osteogenic induction media for 21 days according to the manufacturer's instructions (Cyagen Biosciences, China), after which alizarin red staining was performed to visualize calcium deposits.

Techniques: BrdU Staining, Staining, Expressing

Journal:

Article Title: Structural basis of Semaphorin-Plexin recognition and viral mimicry from Sema7A and A39R complexes with PlexinC1

doi: 10.1016/j.cell.2010.07.040

Figure Lengend Snippet: (A) Domain diagram of PlexinC1, Sema7A and A39R and the constructs used in characterization. The cartoon key for the diagrams is shown to the right.

Article Snippet: The viral Semaphorins probably play an immunomodulatory role by mimicking Sema7A; an antibody against PlexinC1 inhibits A39R-induced induction of inflammatory cytokines by monocytes ( Comeau et al., 1998 ).

Techniques: Construct

Journal:

Article Title: Structural basis of Semaphorin-Plexin recognition and viral mimicry from Sema7A and A39R complexes with PlexinC1

doi: 10.1016/j.cell.2010.07.040

Figure Lengend Snippet: (A) Ribbon models of the Sema7A/PlexinC1-SemaPSI complex in front view (left) and side view (right), with the Sema7A protomers colored in cyan and blue, and the PlexinC1-SemaPSI protomers in pink and magenta. The N-linked glycans are depicted as sticks with carbon atoms colored in green. A cartoon of a membrane is drawn above and below the complex to indicate where the respective proteins would be attached to the cell surfaces.

Article Snippet: The viral Semaphorins probably play an immunomodulatory role by mimicking Sema7A; an antibody against PlexinC1 inhibits A39R-induced induction of inflammatory cytokines by monocytes ( Comeau et al., 1998 ).

Techniques: Membrane

Journal:

Article Title: Structural basis of Semaphorin-Plexin recognition and viral mimicry from Sema7A and A39R complexes with PlexinC1

doi: 10.1016/j.cell.2010.07.040

Figure Lengend Snippet: (A) The overall structure of an interacting pair of Sema7A and PlexinC1-SemaPSI protomers, highlighting the structural elements involved in their binding, colored cyan for Sema7A and pink for PlexinC1-SemaPSI.

Article Snippet: The viral Semaphorins probably play an immunomodulatory role by mimicking Sema7A; an antibody against PlexinC1 inhibits A39R-induced induction of inflammatory cytokines by monocytes ( Comeau et al., 1998 ).

Techniques: Binding Assay

Journal:

Article Title: Structural basis of Semaphorin-Plexin recognition and viral mimicry from Sema7A and A39R complexes with PlexinC1

doi: 10.1016/j.cell.2010.07.040

Figure Lengend Snippet: (A) Structural superposition of the A39R/PlexinC1-SemaPSI and Sema7A/PlexinC1-SemaPSI complexes indicating the closely related docking modes of the viral and mammalian Semaphorins to the PlexinC1 receptor. The complexes have been aligned on the PlexinC1 component in order to visualize the respective overlap of the two different Semaphorins. The A39R is in yellow, Sema7A in cyan.

Article Snippet: The viral Semaphorins probably play an immunomodulatory role by mimicking Sema7A; an antibody against PlexinC1 inhibits A39R-induced induction of inflammatory cytokines by monocytes ( Comeau et al., 1998 ).

Techniques:

Journal:

Article Title: Structural basis of Semaphorin-Plexin recognition and viral mimicry from Sema7A and A39R complexes with PlexinC1

doi: 10.1016/j.cell.2010.07.040

Figure Lengend Snippet: (A), (B), (C) and (D) Stick models of the isolated 4c-4d loops of Sema7A (cyan), A39R (yellow), Sema4D (green), and Sema3A (pink), showing its conserved conformation at the bases (top) and the mid-points of the loops, and variable conformation at the tips of the loops (bottom).

Article Snippet: The viral Semaphorins probably play an immunomodulatory role by mimicking Sema7A; an antibody against PlexinC1 inhibits A39R-induced induction of inflammatory cytokines by monocytes ( Comeau et al., 1998 ).

Techniques: Isolation

Journal:

Article Title: Structural basis of Semaphorin-Plexin recognition and viral mimicry from Sema7A and A39R complexes with PlexinC1

doi: 10.1016/j.cell.2010.07.040

Figure Lengend Snippet: (A) and (B) The respective PlexinC1-binding surfaces of Sema7A (left, cyan) and A39R (right, yellow). The residues mapped at the interface are colored red for acidic residues, blue for basic residues, gray for polar, non-charged residues, and green for apolar residues.

Article Snippet: The viral Semaphorins probably play an immunomodulatory role by mimicking Sema7A; an antibody against PlexinC1 inhibits A39R-induced induction of inflammatory cytokines by monocytes ( Comeau et al., 1998 ).

Techniques: Binding Assay